5XXA

beta-1,4-mannanase-SeMet-RmMan134A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	49.614, 59.083, 124.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.810 - 1.760
Rwork	0.186
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.810	1.820
High resolution limit [Å]	1.760	1.760
Rmerge	0.086
Number of reflections	36268
<I/σ(I)>	8.1	27
Completeness [%]	98.0	98.5
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-Hcl, tri-Sodium Citrate