5XX9
Crystal structure of Bacterioferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 123.761, 123.761, 172.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.756 - 2.600 |
| R-factor | 0.1629 |
| Rwork | 0.161 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cvt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2733)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.800 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 76305 |
| <I/σ(I)> | 3.1 |
| Completeness [%] | 97.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25% PEG 3000, 0.1M HEPES pH 7.5, 0.2M NaCl |
