5XWX

Crystal structure of the four N-terminal immunoglogulin domains of Sidekick-1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	80
Detector technology	CCD
Collection date	2013-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.851, 118.062, 122.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.515 - 1.550
R-factor	0.2227
Rwork	0.222
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.245
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.550
Number of reflections	131095
<I/σ(I)>	10.07
Completeness [%]	97.1
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris(pH 8.0), 30 % PEG 400