5XWK
Crystal Structure of SPAP, an alkaline phosphatase from Sphingomonas in complex with inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979685 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.990, 86.990, 165.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.000 - 1.901 |
| R-factor | 0.1692 |
| Rwork | 0.167 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q3q |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.101 | 0.572 |
| Number of reflections | 50640 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 99.7 | 95.7 |
| Redundancy | 5.6 | 3.3 |
| CC(1/2) | 0.997 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.8M Ammonium Sulfate, 100mM Tris, pH 7.5 |
