5XWG
Crystal structure of a novel RNA motif that allows for precise positioning of a metal ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91972 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 80.544, 80.544, 80.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.526 - 3.002 |
| R-factor | 0.2177 |
| Rwork | 0.217 |
| R-free | 0.22550 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.439 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.953 | 3.100 |
| High resolution limit [Å] | 3.002 | 3.000 |
| Rmerge | 0.337 | |
| Number of reflections | 3373 | |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 11.1 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium cacodylate, spermine tetrahydrochloride, 2-methyl-2,4-pentanediol, strontium chloride |
