5XW7
Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthase subunit C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.810, 55.620, 165.020 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 47.695 - 3.272 |
| R-factor | 0.2154 |
| Rwork | 0.212 |
| R-free | 0.25650 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.280 | 3.270 |
| Rmerge | 0.690 | |
| Number of reflections | 32542 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 98.6 | 98.2 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | MES, sodium chloride | |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | MES, sodium chloride |
