5XW4

Crystal structure of budding yeast Cdc14p (wild type) in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2009-11-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	82.850, 95.130, 137.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.791 - 1.850
R-factor	0.1792
Rwork	0.178
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohe
RMSD bond length	0.004
RMSD bond angle	0.665
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.791	55.790	1.880
High resolution limit [Å]	1.850	10.130	1.850
Rmerge	0.063	0.036	0.616
Rmeas	0.069	0.039	0.706
Rpim	0.026	0.016	0.337
Number of reflections	91638
<I/σ(I)>	16.5
Completeness [%]	98.1	98.5	82.2
Redundancy	6.7	5.7	4.2
CC(1/2)	0.999	0.998	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Bis-Tris, ammonium sulfate, PEG4000