5XVT

Crystal Structure of Transketolase in complex with hydroxylated TPP from Pichia Stipitis, crystal 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-06
Detector	RAYONIX MX300-HS
Wavelength(s)	0.7
Spacegroup name	C 2 2 21
Unit cell lengths	101.220, 185.340, 99.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.676 - 0.850
R-factor	0.1328
Rwork	0.133
R-free	0.13850
RMSD bond length	0.011
RMSD bond angle	1.315
Data scaling software	XDS
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	0.900
High resolution limit [Å]	0.850	0.850
Rmerge	0.090	0.810
Number of reflections	805990
<I/σ(I)>	10.6
Completeness [%]	99.9
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES, 0.1M NaCl, PEG 400