5XVN

E. far Cas1-Cas2/prespacer binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 41
Unit cell lengths	160.711, 160.711, 187.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.410 - 3.250
R-factor	0.20805
Rwork	0.206
R-free	0.24907
RMSD bond length	0.010
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.410
High resolution limit [Å]	3.250
Number of reflections	71385
<I/σ(I)>	13.9
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.5	100 mM Tris-HCl, pH 8.5, and 8% (w/v) PEG 8000