5XVM

Sterol 3-beta-glucosyltransferase (ugt51) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	68.859, 83.571, 151.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.010 - 2.770
R-factor	0.27225
Rwork	0.270
R-free	0.28826
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gl5
RMSD bond length	0.011
RMSD bond angle	1.494
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.150	2.820
High resolution limit [Å]	2.770	2.770
Number of reflections	22991
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)		0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	PEG 3350, magnesium chloride, Tris pH8.5