5XVL

Crystal structure of AL2 PAL domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-03
Detector	RAYONIX MX-225
Wavelength(s)	0.97946, 1.0093, 1.0095, 1.0332
Spacegroup name	P 65 2 2
Unit cell lengths	68.433, 68.433, 111.367
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.632 - 1.629
R-factor	0.1775
Rwork	0.175
R-free	0.20330
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.915
Data reduction software	HKL-2000
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.629
Number of reflections	19891
<I/σ(I)>	70.8
Completeness [%]	99.6
Redundancy	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	289	1.9 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6