5XUG

Complex structure(RmMan134A-M5).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-01-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	44.501, 58.487, 105.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.200 - 2.310
Rwork	0.193
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev-2474-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200
High resolution limit [Å]	2.310	2.310
Rmerge	0.149
Number of reflections	11446
<I/σ(I)>	5.1
Completeness [%]	91.0	91
Redundancy	9.5	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-Hcl, tri-Sodium Citrate