5XUF
Crystal Structure of Transketolase in complex with hydroxylated TPP from Pichia Stipitis, crystal I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-10 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.7 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.205, 185.174, 99.099 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.348 - 0.880 |
R-factor | 0.1289 |
Rwork | 0.129 |
R-free | 0.13650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hyv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.301 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 0.910 |
High resolution limit [Å] | 0.880 | 0.880 |
Rmerge | 0.070 | 0.880 |
Number of reflections | 722426 | 71932 |
<I/σ(I)> | 20.7 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES, 0.1M NaCl, PEG 400 |