5XUB
The citrate-bound trimer of chemoreceptor MCP2201 ligand binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | H 3 |
| Unit cell lengths | 49.400, 49.400, 406.342 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.864 - 2.500 |
| R-factor | 0.2087 |
| Rwork | 0.203 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5XUD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.983 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.150 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.152 | 0.291 |
| Number of reflections | 12095 | 1744 |
| <I/σ(I)> | 4.3 | 2.2 |
| Completeness [%] | 94.5 | 94 |
| Redundancy | 2.3 | 1.9 |
| CC(1/2) | 0.953 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Sodium citrate pH 4.4, 14% PEG 400, 13% Ethylene glycol |
