5XTJ

Mannanase(RmMan134A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	49.259, 59.350, 123.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.700 - 2.220
Rwork	0.173
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev-2474-000)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.700
High resolution limit [Å]	2.200
Rmerge	0.080
Number of reflections	17836
<I/σ(I)>	8.5
Completeness [%]	97.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-Hcl, tri-Sodium Citrate