5XT0
Crystal Structure of Transketolase in complex with TPP intermediate VIII from Pichia Stipitis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-11 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.215, 184.126, 98.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.566 - 1.150 |
R-factor | 0.1228 |
Rwork | 0.122 |
R-free | 0.13460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hyv |
RMSD bond length | 0.013 |
RMSD bond angle | 1.437 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 1.150 | |
Rmerge | 0.052 | 0.316 |
Number of reflections | 320065 | |
<I/σ(I)> | 21.2 | |
Completeness [%] | 99.0 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES, 0.1M NaCl, PEG 400 |