5XSS
XylFII molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.541, 68.059, 76.677 |
| Unit cell angles | 89.89, 65.15, 87.33 |
Refinement procedure
| Resolution | 30.782 - 2.093 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.21440 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.121 |
| High resolution limit [Å] | 2.093 | 2.093 |
| Number of reflections | 70577 | |
| <I/σ(I)> | 15.8 | 7.8 |
| Completeness [%] | 96.8 | 91.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 20% Polyethylene glycol 8000, 0.1 M phosphate-citrate pH 4.2, 0.2 M NaCl |
