5XSO
Crystal structure of full-length FixJ from B. japonicum crystallized in space group C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.849, 79.335, 120.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.674 - 1.778 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20440 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.055 | |
| Rpim | 0.262 | |
| Number of reflections | 53635 | 1502 |
| <I/σ(I)> | 45.8 | 1.9 |
| Completeness [%] | 99.5 | 93.5 |
| Redundancy | 14.3 | 9.9 |
| CC(1/2) | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 8000, PEG 1000, sodium formate, glycerol, Tris-HCl |
