5XSE

Crystal structure of the 2-keto-3-deoxy-6-phosphogluconate aldolase of Zymomonas mobilis ZM4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	63.695, 83.025, 117.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.351 - 1.799
R-factor	0.1921
Rwork	0.190
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3onp
RMSD bond length	0.005
RMSD bond angle	0.707
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.799	1.800
Number of reflections	57557
<I/σ(I)>	17	2.2
Completeness [%]	98.5	95.8
Redundancy	8.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	25%(w/v) polyethylene glycol 8,000, 0.1M Bis-tris (pH 5.5)