5XRW
Crystal structure of flagellar motor switch complex from H. pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.210, 49.210, 52.370 |
| Unit cell angles | 69.34, 82.98, 76.01 |
Refinement procedure
| Resolution | 25.374 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.217 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.420 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 13192 |
| <I/σ(I)> | 6 |
| Completeness [%] | 87.3 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Sodium acetate, 0.1M Tris HCl pH 8.5 and 30% PEG4000 |
