5XRT

Crystal structure of A/Minnesota/11/2010 (H3N2) influenza virus hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.033
Spacegroup name	I 21 3
Unit cell lengths	292.782, 292.782, 292.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.212 - 3.150
R-factor	0.2013
Rwork	0.199
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp2
RMSD bond length	0.005
RMSD bond angle	0.896
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.212	3.200
High resolution limit [Å]	3.150	3.150
Rmerge	0.175	0.869
Rpim	0.051	0.677
Number of reflections	82265	4534
<I/σ(I)>	22.4
Completeness [%]	100.0	100
Redundancy	18.9	17.2
CC(1/2)	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M calcium acetate, 18% (w/v) polyethylene glycol PEG 8000, 0.1 M sodium cacodylate (pH 6.5)