5XRM

Galectin-10/Charcot-Leyden crystal protein variant W72A crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9
Spacegroup name	P 65 2 2
Unit cell lengths	48.830, 48.830, 260.335
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.590 - 1.998
R-factor	0.1734
Rwork	0.170
R-free	0.20780
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.590
High resolution limit [Å]	1.998
Number of reflections	13511
<I/σ(I)>	34.2
Completeness [%]	99.3
Redundancy	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bis-Tris