5XRL

Galectin-10/Charcot-Leyden crystal protein variant N65A crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9
Spacegroup name	P 65 2 2
Unit cell lengths	49.057, 49.057, 259.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.654 - 2.004
R-factor	0.1744
Rwork	0.171
R-free	0.20890
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.654
High resolution limit [Å]	2.000
Number of reflections	13413
<I/σ(I)>	14.2
Completeness [%]	99.0
Redundancy	17.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bis-Tris