5XRC

A Trimodular GH5_4 Subfamily Endoglucanase Structure with Large Unit Cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979065
Spacegroup name	I 4 2 2
Unit cell lengths	120.144, 120.144, 615.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.970 - 2.900
R-factor	0.2788
Rwork	0.276
R-free	0.33210
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	153.834	2.959
High resolution limit [Å]	2.900	2.900
Number of reflections	50812
<I/σ(I)>	8.21
Completeness [%]	98.7
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289.15	1.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6