5XR4

Crystal structure of RabA1a in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	36.708, 70.407, 68.040
Unit cell angles	90.00, 97.21, 90.00

Refinement procedure

Resolution	27.981 - 2.800
R-factor	0.1931
Rwork	0.185
R-free	0.26940
RMSD bond length	0.008
RMSD bond angle	1.099
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	8840
<I/σ(I)>	19.2
Completeness [%]	99.5
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Bis Tris (pH5.5), 0.2 M Ammonium sulfate, 26% PEG 3350