5XR0
Crystal structure of Notched-fin eelpout type III antifreeze protein A20T mutant (NFE6, AFP), P21 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.470, 33.319, 34.349 |
| Unit cell angles | 90.00, 111.97, 90.00 |
Refinement procedure
| Resolution | 31.860 - 0.980 |
| R-factor | 0.11 |
| Rwork | 0.109 |
| R-free | 0.12100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xqn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.020 |
| High resolution limit [Å] | 0.940 | 0.980 |
| Rmerge | 0.041 | 0.236 |
| Number of reflections | 37226 | |
| <I/σ(I)> | 11.9 | 4.8 |
| Completeness [%] | 94.7 | 97.9 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5 M Ammonium sulfate, 0.1 M Na-Citrate pH5.0 |
