5XQP
Crystal structure of Notched-fin eelpout type III antifreeze protein (NFE6, AFP), P212121 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.905 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.553, 75.950, 108.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.170 - 1.000 |
| R-factor | 0.112 |
| Rwork | 0.111 |
| R-free | 0.12500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xqn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.495 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.036 | 0.520 |
| Number of reflections | 167949 | |
| <I/σ(I)> | 36.3 | 2.5 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.5M Ammonium sulfate, 0.1M Na-Citrate pH5.0 |
