5XQL

Crystal structure of a Pseudomonas aeruginosa transcriptional regulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	I 4 2 2
Unit cell lengths	135.944, 135.944, 95.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.051 - 2.494
R-factor	0.2345
Rwork	0.232
R-free	0.27450
RMSD bond length	0.006
RMSD bond angle	0.919
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_2747)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.070
High resolution limit [Å]	2.490
Number of reflections	12920
<I/σ(I)>	20.8
Completeness [%]	81.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293.15	0.15 M Mg Cl2, 0.1 M Bis-Tris pH 6.5, 25% PEG3350