5XQJ

Crystal structure of a PL 26 exo-rhamnogalacturonan lyase from Penicillium chrysogenum complexed with unsaturated galacturonosyl rhamnose substituted with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-13
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	167.616, 171.800, 342.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	171.170 - 2.750
R-factor	0.19921
Rwork	0.196
R-free	0.26197
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xq3
RMSD bond length	0.014
RMSD bond angle	1.683
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	171.170	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.087	0.353
Number of reflections	255645	12654
<I/σ(I)>	29.5	6.57
Completeness [%]	100.0	100
Redundancy	7.4	7.5
CC(1/2)		0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	3.3M Sodium acetate