5XQJ
Crystal structure of a PL 26 exo-rhamnogalacturonan lyase from Penicillium chrysogenum complexed with unsaturated galacturonosyl rhamnose substituted with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 167.616, 171.800, 342.337 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 171.170 - 2.750 |
| R-factor | 0.19921 |
| Rwork | 0.196 |
| R-free | 0.26197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xq3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.683 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 171.170 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.087 | 0.353 |
| Number of reflections | 255645 | 12654 |
| <I/σ(I)> | 29.5 | 6.57 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.5 |
| CC(1/2) | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 3.3M Sodium acetate |
