5XQI

Crystal structure of full-length human Rogdi

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	H 3
Unit cell lengths	169.372, 169.372, 220.323
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.170 - 2.800
Rwork	0.219
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xqh
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.551
Number of reflections	57790
<I/σ(I)>	44.6	5.7
Completeness [%]	99.8	100
Redundancy	9.1	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2M sodium chloride, 130mM magnesium chloride, 100mM TRIS pH 7.0