5XQG
Crystal structure of a PL 26 exo-rhamnogalacturonan lyase from Penicillium chrysogenum complexed with unsaturated galacturonosyl rhamnose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 167.878, 171.935, 342.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 171.190 - 2.740 |
| R-factor | 0.19088 |
| Rwork | 0.188 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xq3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 171.190 | 2.790 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.083 | 0.316 |
| Number of reflections | 257925 | 6996 |
| <I/σ(I)> | 25.9 | 5.93 |
| Completeness [%] | 99.5 | 98.5 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 3.3M Sodium acetate |
