5XQG

Crystal structure of a PL 26 exo-rhamnogalacturonan lyase from Penicillium chrysogenum complexed with unsaturated galacturonosyl rhamnose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-13
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	167.878, 171.935, 342.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	171.190 - 2.740
R-factor	0.19088
Rwork	0.188
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xq3
RMSD bond length	0.014
RMSD bond angle	1.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	171.190	2.790
High resolution limit [Å]	2.740	2.740
Rmerge	0.083	0.316
Number of reflections	257925	6996
<I/σ(I)>	25.9	5.93
Completeness [%]	99.5	98.5
Redundancy	6.6	6
CC(1/2)		0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	3.3M Sodium acetate