5XQD
Crystal structure of Human Hsp90 with FS2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97892 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.610, 89.238, 99.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 1.600 |
R-factor | 0.1875 |
Rwork | 0.187 |
R-free | 0.19970 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.033 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.211 |
High resolution limit [Å] | 1.597 |
Number of reflections | 39021 |
<I/σ(I)> | 10.3 |
Completeness [%] | 99.8 |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20%(w/v) PEG 2000 monomethyl ether, 200mM magnesium chloride, 100mM sodium cacodylate, pH 6.5, |