5XQ0

Structural basis of kindlin-mediated integrin recognition and activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	75.866, 75.866, 384.219
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.858 - 2.750
R-factor	0.2341
Rwork	0.232
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xpz
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.086	0.048	0.788
Rmeas	0.098	0.053	0.895
Rpim	0.045	0.022	0.413
Total number of observations	139053
Number of reflections	34443
<I/σ(I)>	12
Completeness [%]	98.7	97.5	99.8
Redundancy	4	5	4.4
CC(1/2)		0.997	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M potassium chloride, 0.05 M HEPES pH 7.5, 35% v/v pentaerythritol propoxylate