5XPJ
Crystal Structure of Periplasmic glucose binding protein ppGBP deletion mutant- Del-ppGBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-09-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.200, 137.600, 54.200 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 37.220 - 2.500 |
| R-factor | 0.21577 |
| Rwork | 0.213 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dvi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.787 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 25721 | 9773 |
| <I/σ(I)> | 6.16 | |
| Completeness [%] | 95.4 | 89.9 |
| Redundancy | 3.64 | 3.74 |
| CC(1/2) | 0.980 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M Bis-Tris, pH 6.5, 25% PEG 3350 |
