5XPE
Neutron structure of the T26H mutant of T4 lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2015-05-11 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.230, 61.230, 96.791 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.025 - 2.090 |
R-factor | 0.2275 |
Rwork | 0.225 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qt8 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.432 |
Data scaling software | SCALA (3.2.25) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.505 | 17.107 | 1.710 |
High resolution limit [Å] | 1.648 | 6.640 | 1.650 |
Rmerge | 0.053 | 0.096 | 0.527 |
Rmeas | 0.057 | 0.113 | 0.562 |
Rpim | 0.018 | 0.058 | 0.192 |
Number of reflections | 25927 | 2538 | |
<I/σ(I)> | 65.2 | 5.5 | 5.3 |
Completeness [%] | 99.9 | 86 | 100 |
Redundancy | 10.8 | 3 | 8.5 |
CC(1/2) | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium-potassium phosphate, Sodium chloride, 1,6-Hexanediol |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium-potassium phosphate, Sodium chloride, 1,6-Hexanediol |