5XPC
Crystal Structure of Drep4 CIDE domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-05-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.081, 76.588, 174.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.068 - 1.902 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xpb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.805 |
| Data reduction software | PHENIX ((1.10.1_2155)) |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((1.10.1_2155)) |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.037 | |
| Number of reflections | 56853 | 5578 |
| <I/σ(I)> | 0.352 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M tris-hydrochloride pH 8.5, 20 mM ammonium phosphate |
