5XPB
Crystal Structure of Selenomethionine labelled Drep4 CIDE domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-07-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.511, 103.722, 103.820 |
| Unit cell angles | 102.46, 105.81, 105.77 |
Refinement procedure
| Resolution | 29.830 - 2.996 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.26340 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.338 |
| Data reduction software | PHENIX (1.9_1690) |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1690) |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.103 |
| High resolution limit [Å] | 2.996 | 2.996 |
| Rmerge | 0.067 | 0.322 |
| Number of reflections | 38172 | 3334 |
| <I/σ(I)> | 13.03 | 1.9 |
| Completeness [%] | 81.6 | 65 |
| Redundancy | 1.6 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M tris-hydrochloride pH 8.5, 20 mM ammonium phosphate monobasic |
