5XOS
Crystal structure of HLA-B35 in complex with a pepetide antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.702, 81.915, 110.084 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.726 - 1.697 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.18380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a9e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.784 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.697 | 1.697 |
| Rmerge | 0.077 | |
| Number of reflections | 51567 | |
| <I/σ(I)> | 25.433 | 5.981 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1M MES monohydrate, pH 6.5, 12%(w/v) PEG20000 |
