5XOR

Crystal structure of N-terminal replicase protein of porcine circovirus type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	84.932, 71.213, 131.718
Unit cell angles	90.00, 104.34, 90.00

Refinement procedure

Resolution	42.340 - 2.699
R-factor	0.2306
Rwork	0.224
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hw0
RMSD bond length	0.010
RMSD bond angle	1.154
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	46536
<I/σ(I)>	12.2
Completeness [%]	97.9
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1M HEPES sodium pH 8.0, 0.45 M sodium citrate tribasic dihydrate, 50% (+/-)-2-Methyl-2,4-pentanediol