5XOP
Crystal Structure of N-terminal domain EhCaBP1 EF-2 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.690, 101.359, 107.475 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.740 - 1.900 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.740 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.888 | |
| Number of reflections | 39381 | 39381 |
| <I/σ(I)> | 33 | 1.9 |
| Completeness [%] | 98.7 | 92.3 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 58%-63% MPD, 5mM Cacl2 |
