5XOO

The structure of hydra Fam20 with sugar

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	80.601, 123.580, 143.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.227 - 2.850
R-factor	0.2008
Rwork	0.200
R-free	0.21710
RMSD bond length	0.004
RMSD bond angle	0.644
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	34403
<I/σ(I)>	2
Completeness [%]	99.8
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	291	1.8 M NaH2PO4/ K2HPO4, pH 6.9, 10 mM MgCl2, 5 mM AMP-PNP, and 2 mM Gal-Xyl-O-benzyl