5XOF
Crystal structure of human paired immunoglobulin-like type 2 receptor alpha with synthesized glycopeptide I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-25 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.660, 63.009, 78.599 |
| Unit cell angles | 90.00, 108.34, 90.00 |
Refinement procedure
| Resolution | 40.050 - 1.963 |
| Rwork | 0.214 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wv0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.105 | 0.322 |
| Number of reflections | 36271 | |
| <I/σ(I)> | 20.9 | 5.5 |
| Completeness [%] | 99.4 | 96.35 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris-HCl pH8.5, 25% (w/v) PEG 6000 |
