5XO6
Crystal structure of a novel ZEN lactonase mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-16 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.012, 96.887, 101.895 |
| Unit cell angles | 88.96, 87.70, 87.96 |
Refinement procedure
| Resolution | 24.810 - 2.380 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wzl |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.756 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.460 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.058 | 0.370 |
| Number of reflections | 114582 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 98.5 | 96.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Ammonium Sulfate, 0.085M Sodium Cacodylate pH 6.5, 25-28%(w/v) Polyethylene Glycol 8000 and 15%(v/v) Glycerol |
