5XM9
Crystal structure of AsfvPolX in complex with DNA enzyme.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.940, 118.878, 235.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.719 - 3.053 |
| R-factor | 0.2188 |
| Rwork | 0.215 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hrb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.110 | 0.350 |
| Number of reflections | 26143 | 2408 |
| <I/σ(I)> | 20.4 | 1.9 |
| Completeness [%] | 97.5 | 90.5 |
| Redundancy | 8.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M di-ammonium hydrogen phosphate and 20% PEG 3350 |
