5XM1
The mouse nucleosome structure containing H2A, H2B type3-A, H3mm7, and H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.550, 109.380, 176.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.875 - 3.450 |
| R-factor | 0.2037 |
| Rwork | 0.200 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3av2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data scaling software | HKL-2000 (704w) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.570 |
| High resolution limit [Å] | 3.450 | 7.430 | 3.450 |
| Rmerge | 0.097 | 0.051 | 0.426 |
| Total number of observations | 169255 | ||
| Number of reflections | 28747 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 99.3 | 98.5 | 98.9 |
| Redundancy | 5.9 | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
