5XLN

Crystal structure of the TRS_UNE-T and 4EHP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	49.369, 60.569, 85.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.815 - 1.900
R-factor	0.1628
Rwork	0.161
R-free	0.19640
RMSD bond length	0.007
RMSD bond angle	0.992
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	20718
<I/σ(I)>	21.1
Completeness [%]	99.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	295	20% PEG 8000, 0.1 M CHES (pH 9.5)