5XLK
Crystal structure of the flagellar cap protein FliD D2-D3 domains from Serratia marcescens in Space group I422
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00004 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 121.362, 121.362, 249.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| R-factor | 0.24069 |
| Rwork | 0.238 |
| R-free | 0.28654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xlj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.125 | 0.476 |
| Number of reflections | 17805 | |
| <I/σ(I)> | 25.9 | |
| Completeness [%] | 96.9 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 2.5 M sodium chloride, 0.1 M imidazole, pH 8.0, and 0.2 M zinc acetate |
