5XL5
The structure of hemagglutinin Q226L mutant from an avian-origin H4N6 influenza virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.549, 100.549, 685.737 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.820 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ha0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.812 | |
| Number of reflections | 69076 | |
| <I/σ(I)> | 18.8 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.1 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 291 | 0.2 M Trimethylamine N-oxide dehydrate, 0.1 M Tris (pH 8.4), and 18% PEG MME 2000 |
