5XL1
The structure of hemagglutinin from an avian-origin H4N6 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.476, 100.476, 685.126 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.420 - 2.300 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ha0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.016 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.686 | |
Number of reflections | 60221 | |
<I/σ(I)> | 17.5 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2 M Trimethylamine N-oxide dehydrate, 0.1 M Tris (pH 8.0), and 20% PEG MME 2000 |