5XKJ

Crystal structure of plant receptor ERL1-TMM in complexe with EPF2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-30
Detector	BRUKER SMART 6000
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	66.154, 65.601, 142.496
Unit cell angles	102.86, 97.60, 93.58

Refinement procedure

Resolution	46.186 - 3.475
R-factor	0.2555
Rwork	0.254
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mn8
RMSD bond length	0.005
RMSD bond angle	0.850
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.599
High resolution limit [Å]	3.450	3.475
Number of reflections	29462
<I/σ(I)>	6.8
Completeness [%]	93.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 mM Ammonium citrate tribasic pH 7.0, 10% Polyethylene glycol 3350